Sam and Ella

The effect of an enteric infection caused by Salmonella was assessed in this relationship. LAB attached itself to the lining of the gut, were it is usually found in abundance. When a Salmonella infection occurs, the two organisms are now forced to compete for the same binding sites on the gut lining. During Subject B’s infection there were significant quantities of LAB found in the subjects stool samples as a result. The quantity of LAB was compared to that of Salmonella, within the stool samples by observing the amount of LAB present. The more LAB present within the sample would indicate that Salmonella is outcompeting the LAB for binding sites in the GIT. This selection indicates that one pathogenic organism has the ability to change the entire composition of the microbiome. The competition between Salmonella and LAB was clearly demonstrated in the change of pitch. As the pitch increases the relative abundance of LAB also increased. Days 150, 151, 153, 155, 156, 157, 158 and 159 were selected as they were the only days that illness was reported by the subject in the corresponding metadata files, it is important to note that only data pertaining to subject B was utilised for this analysis. The relative abundance OTUs of Lactobacillales was used to create a melody, revealing a clear pattern created over two bars in 4/4 time. This meant that a melody made up of 8 notes was generated in order to make a repetitive tune that would be simpler to learn. Again, random chords were chosen, in this instance based on an American folk song titled “Saint James Infirmary”.