Choir Performance

https://www.youtube.com/watch?v=gJTBGtZIHFg

Nucleotide Hits

Nucleotide Hits is based on particular DNA sequences from the 16S ribosome of the six most abundant orders present in subject B. The six most abundant orders present within subject B included; Clostridiales, Bacteroidales, Bifidobacteriales, Erysipelotrichales, Lactobacillales and Burkholderiales.
Days 17 through 25 were selected for subject B. This period was chosen as no irregularities in the data sets were observed during this time. The melodies were randomly generated to create the songs for this relationship. The chord progressions were based on the list of the above relative abundance orders of bacteria. The chords were randomly selected and was based on Robert Palmers song “Addicted to Love”.

 

Home is where the biome is

The relationship between the sensitivity of the human microbiome to the environment was assessed in this piece. It is important to note that only data from subject A was utilized during this analysis. Three time intervals were chosen to determine whether or not the relationship indicated any significance. Firstly a period was selected before the subject travelled, which included days 53 through 60. Secondly a period was selected where the subject was traveling abroad which included days 113 through 120. It is of importance to note that there was a period during travel where subject A was unwell, but this time period was not explored as a relationship due to the fact that it could not be compared to a time period where subject A was not traveling and unwell. Lastly a period was selected after the subject had returned from said travels which encompassed days 138 through 146.

The relative abundances of the bacterial families clearly indicated a shift in the composition of the orders present within the microbiome.

The four most abundant bacterial families over all three time periods were selected, namely: Actinomycetales, Bifidobacteriales, Coriobacteriales, and Lactobacillales. Bacterial data used were selected from high abundance bacteria over the selected time period. The bacterial names also possessed the appropriate quantity of syllables needed to add lyrics to 4/4 time. This meant that once the music had been written the names of the bacteria were suitable for use as lyrics. The chord progression was randomly generated based on Elton John’s “Rocket Man”.

Sam and Ella

The effect of an enteric infection caused by Salmonella was assessed in this relationship. LAB attached itself to the lining of the gut, were it is usually found in abundance. When a Salmonella infection occurs, the two organisms are now forced to compete for the same binding sites on the gut lining. During Subject B’s infection there were significant quantities of LAB found in the subjects stool samples as a result. The quantity of LAB was compared to that of Salmonella, within the stool samples by observing the amount of LAB present. The more LAB present within the sample would indicate that Salmonella is outcompeting the LAB for binding sites in the GIT. This selection indicates that one pathogenic organism has the ability to change the entire composition of the microbiome. The competition between Salmonella and LAB was clearly demonstrated in the change of pitch. As the pitch increases the relative abundance of LAB also increased. Days 150, 151, 153, 155, 156, 157, 158 and 159 were selected as they were the only days that illness was reported by the subject in the corresponding metadata files, it is important to note that only data pertaining to subject B was utilised for this analysis. The relative abundance OTUs of Lactobacillales was used to create a melody, revealing a clear pattern created over two bars in 4/4 time. This meant that a melody made up of 8 notes was generated in order to make a repetitive tune that would be simpler to learn. Again, random chords were chosen, in this instance based on an American folk song titled “Saint James Infirmary”.